Yeast strain development
Our engineered yeast strain cV-110 features a combination of two heterologous enzymes of bacterial origin, xylose isomerase and aldose 1-epimerase, as well as the upregulation of the following enzymes as part of the pentose-phosphate pathway: ribose-5-phosphate, transketolase, and transaldolase.
This unique combination of genetic alterations allows cV-110 to:
- Simultaneously utilize C6 and C5 sugars in an efficient manner, also at low xylose concentration
- Operate with a balanced cofactor requirement
- Convert xylose anomers and xylulose into pentose-phosphate pathway intermediates at a high rate
- Show a robust performance and high ethanol yields on lignocellulosic-based feedstocks, such as agricultural crop residues and wood & paper mill discards.
Fed-batch fermentations yielding 95% or more have previously shown scalability from 2 L laboratory scale to 270.000 L demonstration scale, and vice versa
Yeast Propagation and Storage
In order to generate viable yeast cells for e.g. inoculation, a protocol for efficient yeast propagation was developed, resulting in cell concentrations above 100 g/L.
In parallel, a protocol for drying yeast cells was established to allow for easy delivery of dry yeast batches for e.g. pitching guaranteeing reproducible cell viability and performance after hydration.
Protocols and provision of dry yeast samples on request